Search results for " ISSR"
showing 5 items of 5 documents
Recupero, conservazione ed analisi genetica della collezione di agrumi dell’Orto Botanico di Palermo
2007
Genetic diversity in Sicilian populations of Quercus ilex (Fagaceae)
2013
Raimondo, F. M., Scialabba, A., Guarino, R. & Spallino, R. E.: Genetic diversity in Sicilian populations of Quercus ilex (Fagaceae). — Fl. Medit. 23: 245-253. 2013. — ISSN: 1120-4052 printed, 2240-4538 online. Recent studies on the genetic structure of the Italian populations of Quercus ilex demonstrated their high diversity, particularly in the Sicilian metapopulation. In order to check if the holm oak stands in the mountains of NW Sicily could have some relationship with Q. ilex subsp. ballota (distributed in Spain and NW Africa), 26 specimens from the Sicilian most distinctive Q. ilex populations have been compared with some representative populations of the Italian Peninsula, North Afri…
Genetic analysis of Citrus aurantium L. (Rutaceae) cultivars by ISSR molecular markers
2011
Eight cultivars of Citrus aurantium L., showing peculiar morphological traits of the fruits and leaves, were analyzed genetically. Inter simple sequence repeat (ISSR) was chosen as molecular markers because they represent a highly efficient system for investigating variability at intraspecific level. The particular morphological traits were discussed, the genetic identity and distance matrix based on Nei index was calculated, and the unweighted pair group method with arithmetic mean (UPGMA) dendrogram was generated. A total of 53 clearly distinguished DNA bands were considered for the ISSR analysis of which 24 were polymorphic. A basic C. aurantium fingerprinting pattern was obtained. The I…
Hybridization in Capparis spinosa L.: Molecular and morphological evidence from a Mediterranean island complex
2014
Inter-Simple Sequence Repeat (ISSR) molecular markers and morphological analysis were used in order to characterize wild populations and cultivated forms of orphan crop species Capparis spinosa L. in a Mediterranean island complex. Nineteen wild populations belonging to two different subspecies, C. spinosa subsp. spinosa and subsp. rupestris, were sampled in different environments in Sicily and the surrounding islets Lampedusa, Pantelleria and Salina. Different biotypes cultivated in Pantelleria and Salina were analyzed. Six ISSR primers were selected for genetic characterization, and all clear and reproducible bands were scored and analyzed. Among the 47 ISSR bands obtained, 97.5% were pol…
Morpho-agronomic and genetic diversity among twelve sicilian agro-ecotypes of lentil (Lens culinaris)
2015
Although Sicily is relatively small (about 25000 km2), it accounts for several agro-ecotypes of lentil (Lens culinaris Medik.), for many of which no data on morphological, agronomic, and organoleptic characteristics are available to date. Thus, experiments were performed to characterize 12 lentil agro-ecotypes from different areas of Sicily, including some small islands surrounding the main island, and to assess the extent of genetic diversity (by means of six inter-simple sequence repeat [ISSR] primers). A famous agro-ecotype from central Italy (Castelluccio di Norcia) and two commercial varieties from Canada (Eston and Laird) were also included in the study. The results showed a large deg…